Microbial transformation of quinic acid to shikimic acid by Bacillus megaterium

Generation of aroE overexpression mutant of Bacillus megaterium for the production of shikimic acid

BACKGROUND Shikimic acid, the sole chemical building block for the antiviral drug oseltamivir (Tamiflu®), is one of the potent pharmaceutical intermediates with three chiral centers. Here we report a metabolically engineered recombinant Bacillus megaterium strain with aroE (shikimate dehydrogenase) overexpression for the production of shikimic acid. RESULTS In a 7 L bioreactor, 4.2 g/L shikim...

Biosynthesis of dipicolinic acid in Bacillus megaterium.

Very little is known of the precursors of the dipicolinic acid (pyridine-2,6-dicarboxylic acid) in bacterial spores. C'4-2,6-diaminopimelic acid was shown to contribute to the formation of dipicolinic acid in Bacillus cereus var. mycoides (Perry and Foster, 1955; Foster, 1956) but the efficiency of the conversion was low, possibly because of a slow penetration of the cells by the exogenously su...

Synthesis of Regioselectively Protected Protocatechuic Acid Derivatives by Biomimetic Transformation of Quinic Acid

3and 4-monoesterified protocatechuic acid methyl ester (5 and 7 respectively) have been synthesised from quinic acid by a high yields biomimetic rearrangement.

A direct, biomass-based synthesis of benzoic acid: formic acid-mediated deoxygenation of the glucose-derived materials quinic acid and shikimic acid.

Bioremedial Application of Bacillus Megaterium PMS82 in Microbial Degradation of Acid Orange Dye

A potential bacterial strain PMS82, capable of degrading an azo dye Acid orange as a sole source of carbon was isolated from common effluent treatment plant of Ankleshwar, India. The 16S rDNA sequence and phenotypic characteristics indicated that an isolated organism as Bacillus megaterium PMS82. This strain exhibited complete decolorization of Acid orange (100 mg/L) within 16 h, while maximall...